Heart Infusion Agar with Horse Serum and Fresh Yeast Extract

[所属分类:培养基配方] [发布时间:2021-3-31] [发布人: ] [阅读次数:] [返回]

Heart Infusion Agar

with Horse Serum and Fresh Yeast Extract

培养基配方

Composition per 930.0mL:
Heart infusion agar ................................................................720.0mL
Horse serum, unheated...........................................................200.0mL
Fresh yeast extract solution .....................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Heart Infusion Agar:
Composition per liter:
Beef heart, infusion from..........................................................500.0g
Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of Heart Infusion Agar:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8.
Preparation of Medium:  To 720.0mL of sterile cooled heart infusion broth, aseptically add 200.0mL of horse serum and 10.0mL of fresh yeast extract solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Mycoplasma equigenitalium and Mycoplasma subdolum.