Heart Infusion Broth with Additives for Staphylococcus

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth

with Additives for Staphylococcus

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................500.0g
NaCl...........................................................................................30.0 g
Tryptose ......................................................................................10.0g
Horse serum, inactivated........................................................100.0mL
Penicillin solution....................................................................10.0mL
Fresh yeast extract solution........................................................5.0mL
pH 7.4 ± 0.2 at 25°C
Penicillin Solution:
Composition per 10.0mL:
Penicillin...........................................................................1,000,000U
Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................10.0g
Preparation of Fresh Yeast Extract Solution : Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8.
Preparation of Medium: Add components—except horse serum, fresh yeast extract solution, and penicillin solution—to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Staphylococcus species.