Heart Infusion Broth with 0.1% Glucose (ATCC Medium 544)

[所属分类:培养基配方] [发布时间:2021-4-1] [发布人: ] [阅读次数:] [返回]

Heart Infusion Broth with 0.1% Glucose

(ATCC Medium 544)

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Glucose.........................................................................................1.0g
pH 7.4 ± 0.2 at 25°C
Source:  Heart infusion broth without glucose is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Heart Infusion Broth with Glucose and Antibiotics
Composition per liter:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Antibiotic inhibitor solution ....................................................10.0mL
Glucose solution ......................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Antibiotic Inhibitor Solution:
Composition per 10.0mL:
Streptomycin sulfate.....................................................................0.1g
Tetracycline·HCl.......................................................................0.025g
Preparation of Antibiotic Inhibitor Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 10.0mL:

D- Glucose......................................................................................1.0g

Preparation of Glucose Solution: Add  D- glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhibitor solution and glucose solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic inhibitor solution and glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Escherichia coli.