Heart Infusion Medium with Fetal Bovine Serum

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Heart Infusion Medium with Fetal Bovine Serum

培养基配方

Composition per liter:
Beef heart, infusion from..........................................................500.0g
Agar ............................................................................................15.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Fetal bovine serum.................................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except fetal bovine serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile fetal bovine serum. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Haemophilus ducreyi.