HHD Medium

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

HHD Medium

培养基配方

Composition per liter:
Agar............................................................................................20.0g
Pancreatic digest of casein..........................................................10.0g
Casamino acids.............................................................................3.0g
Fructose.........................................................................................2.5g
KH 2 PO 4 .........................................................................................2.5g
Papaic digest of soybean meal......................................................1.5g
Tween™ 80...................................................................................1.0g
Yeast extract..................................................................................1.0g
Bromcresol Green solution......................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Green Solution:
Composition per 30.0mL:
Bromcresol Green.........................................................................0.1g
NaOH (0.01N solution)............................................................30.0mL
Preparation of Bromcresol Green Solution: Add Bromcresol Green to 30.0mL of NaOH solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Salmonella species from foods.