HiCrome™ Candida Differential Agar Base, Modified with Candida Selective Supplement

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

HiCrome™ Candida Differential Agar Base, Modified

with Candida Selective Supplement

培养基配方

Composition per liter:
Agar ............................................................................................18.0g
Glucose .......................................................................................10.0g
Peptic digest of animal tissue........................................................5.0g
Malt extract...................................................................................3.0g
Yeast extract..................................................................................3.0g
Chromogenic mixture...................................................................3.0g
Chloramphenicol.........................................................................0.05g
Gentamicin solution.................................................................. 1.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Candida Selective Supplement:
Composition per 10.0mL:
Gentamicin....................................................................................0.1g
Preparation of Candida Selective Supplement: Add gentamicin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except Candida selective supplement, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Do not autoclave. Do not overheat. Cool to 50°C. Aseptically add 10.0mL Candida selective supplement. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use:  For the rapid isolation and identification of Candida species from mixed cultures.