HiCrome™ Coliform Agar with SLS and Novobiocin

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ Coliform Agar with SLS and Novobiocin

培养基配方

Composition per liter:
Agar............................................................................................12.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................3.0g
Peptone, special ............................................................................3.0g
KH 2 PO 4 .........................................................................................1.7g
Sodium pyruvate...........................................................................1.0g
Tryptophan....................................................................................1.0g
Chromogenic mixture...................................................................0.2g
Sodium lauryl sulfate....................................................................0.1g
Novobiocin ................................................................................5.0mg
pH 7.1 ± 0.2 at 25°C
Source: This medium, wihout novobiocin, is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the simultaneous detection of Escherichia coli and total coliforms in water and food samples when a high number of Gram-positive accompanying bacteria are expected.