HiCrome™ E. coli Agar

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ E. coli Agar

培养基配方

Composition per liter:
Casein enzymic hydrolysate.......................................................14.0g
Agar............................................................................................12.0g
Peptone, special ............................................................................5.0g
NaCl..............................................................................................2.4g
Bile salts mixture..........................................................................1.5g
Na 2 HPO 4 .......................................................................................1.0g
NaH 2 PO 4 ......................................................................................0.6g
X-Glucuronide..........................................................................0.075g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the simultaneous detection of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent.