HiCrome™ E. coli Agar A (E. coli Agar A, HiCrome™)

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ E. coli Agar A

(E. coli Agar A, HiCrome™)

培养基配方

Composition per liter:
Casein enzymic hydrolysate .......................................................14.0g
Agar ............................................................................................12.0g
Peptone, special ............................................................................5.0g
NaCl..............................................................................................2.4g
Bile salts mixture..........................................................................1.5g
Na 2 HPO 4 .......................................................................................1.0g
NaH 2 PO 4 .......................................................................................0.6g
X-Glucuronide..........................................................................0.075g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Pour into sterile Petri dishes.
Use: For the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. The chromogenic agent X-glucuronide used in this medium helps to detect glucuronidase activity. E. coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives coloration to the colonies. Bile salts mixture inhibits Gram-positive organisms.