HiCrome™ ECC Agar (ECC Agar, HiCrome™)

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ ECC Agar

(ECC Agar, HiCrome™)

培养基配方

Composition per liter:
Chromogenic mixture.................................................................20.3g
Agar............................................................................................15.0g
Peptone, special ............................................................................5.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 .......................................................................................3.5g
Yeast extract..................................................................................3.0g
Lactose..........................................................................................2.5g
KH 2 PO 4 .........................................................................................1.5g
Neutral Red.................................................................................0.03g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. A differential medium for presumptive identification of E. coli and other coliforms in food samples.  The chromogenic mixture contains two chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme  β -glucuronidase produced by E. coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by the majority of coliforms, including E. coli.