HiCrome™ Enterobacter sakazakii Agar, Modified

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ Enterobacter sakazakii Agar, Modified

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Casein enzymatic hydrolysate ......................................................7.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Sodium deoxycholate....................................................................0.6g
Chromogenic substrate ...............................................................0.15g
Crystal Violet..............................................................................0.02g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the isolation and identification of Enterobacter sakazakii from food and environmental samples. Meets the formulation recommended by ISO Committee for the isolation and identification of Enterobacter sakazakii from milk and milk products.