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培养基配方
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HiCrome™ UTI Agar, Modified
[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]
HiCrome™ UTI Agar, Modified
培养基配方
Composition per liter:Peptic digest of animal tissue .....................................................18.0g
Agar............................................................................................15.0g
Chromogenic mixture...............................................................12.44g
Beef extract...................................................................................6.0g
Casein enzymic hydrolysate.........................................................4.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the differentiation and enumeration of thermotolerant E. coli from water by the membrane filtration method. For the identification, differentiation, and confirmation of enteric bacteria from specimens such as urine, water, or food which may contain large numbers of Proteus species as well as potentially pathogenic Gram-positive organisms.