HiFluoro™ Pseudomonas Agar Base

[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]

HiFluoro™ Pseudomonas Agar Base

培养基配方

Composition per liter:
Pancreatic digest of gelatin.........................................................18.0g
Agar ............................................................................................15.0g
K 2 SO 4 ..........................................................................................10.0g
Fluorogenic mixture....................................................................2.05g
MnCl 2 ............................................................................................1.4g
Cetrimide ......................................................................................0.3g
Glycerol ......................................................................................10.ml
pH 7.2 ± 0.2 at 25°C
Source: This medium, wihout glycerol, is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For the selective isolation of Pseudomonas aeruginosa from clinical and nonclinical specimens by the fluorogenic method.