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High Salt Peptone Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]

High Salt Peptone Yeast Extract Agar

培养基配方

Composition per liter:
NaCl............................................................................................30.0g
Agar............................................................................................15.0g
Peptic digest of animal tissue .....................................................10.0g
Yeast extract..................................................................................6.0g
Meat extract..................................................................................2.0g
Glucose.........................................................................................2.0g
L -Cysteine·HCl·H 2 O.....................................................................0.3g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the confirmation of Vibrio species.
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