HiVeg Hydrolysate Agar with 2.5% Agar

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

HiVeg Hydrolysate Agar with 2.5% Agar

培养基配方

Composition per liter:
Agar............................................................................................25.0g
Plant hydrolysate ..........................................................................5.0g
Plant peptone ................................................................................5.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 ......................................................................................2.5g
Plant infusion................................................................................1.5g
Yeast autolysate ............................................................................1.5g
Glycerol ...................................................................................22.0mL
pH 7.8 ± 0.2 at 25°C
Source: This medium, without glycerol, is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Vibrio cholerae. For the production of cholera vaccine.