Acetogenium Medium

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetogenium Medium
Composition per liter:
Na 2 HPO 4 ·12H 2 O...........................................................................6.1g
NaH 2 PO 4 ·H 2 O...............................................................................4.5g
L -Cysteine·HCl .............................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
NaCl............................................................................................0.45g
NH 4 Cl .........................................................................................0.31g
K 2 HPO 4 .......................................................................................0.22g
KH 2 PO 4 .......................................................................................0.22g
(NH 4 ) 2 SO 4 ..................................................................................0.22g
MgSO 4 ·7H 2 O..............................................................................0.09g
CaCl 2 ·2H 2 O ...............................................................................6.0mg
FeSO 4 ·7H 2 O...............................................................................2.0mg
Resazurin ...................................................................................1.0mg
Trace elements solution...........................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid......................................................................1.5 g
CaCl 2 ·2H 2 O..................................................................................1.0g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
FeSO 4 ·7H 2 O.................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAI(SO 4 ) 2 ·12H 2 O......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution : Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add distilled/deionized water to 1.0L. Add remain-
ing components. Mix thoroughly. Adjust pH to 7.0 with KOH.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Adjust pH to 7.2–7.4 with NaOH. Mix
thoroughly. Gently heat and bring to boiling. Boil for a few minutes.
Allow to cool to room temperature under 80% H 2 + 20% CO 2 . Distrib-
ute into tubes or flasks under 80% H 2 + 20% CO 2 . Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Acetogenium kivui.