HR Antifungal Assay Medium Buffered with MOPS

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

HR Antifungal Assay Medium Buffered with MOPS

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MOPS (3-N-morpholino-
propanesulfonic acid) buffer...............................................34.53g
Glucose .......................................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................2.5g
KH 2 PO 4 .........................................................................................1.0g
NaHCO 3 ........................................................................................1.0g
Glutamine....................................................................................0.58g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.1g
NaCl..............................................................................................0.1g
L- Lysine.......................................................................................0.07g
L- Isoleucine.................................................................................0.05g
L- Leucine.....................................................................................0.05g
L- Threonine.................................................................................0.05g
L- Valine .......................................................................................0.05g
L- Arginine ...................................................................................0.04g
L- Histidine...................................................................................0.02g
L- Methionine...............................................................................0.01g
L- Tryptophan..............................................................................8.2mg
DL- Methionine............................................................................2.0mg
DL- Tryptophan............................................................................2.0mg
Inositol.......................................................................................2.0mg
L- Histidine·HCl..........................................................................1.0mg
H 3 BO 3 ........................................................................................0.5mg
Calcium pantothenate ................................................................0.4mg
MnSO 4 ·H 2 O...............................................................................0.4mg
Niacin.........................................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Riboflavin ..................................................................................0.2mg
Na 2 MoO 3 ....................................................................................0.2mg
KI...............................................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Biotin..........................................................................................2.0μg
Folic acid ....................................................................................2.0μg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except NaHCO 3 and
MOPS buffer, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Add NaHCO 3 and MOPS buffer. Mix thor-
oughly. Adjust pH to 7.0. Bring volume to 1.0L with distilled/deion-
ized water. Filter sterilize.
Use: For testing the effectiveness of antifungal agents against clinical
fungal isolates using the broth dilution susceptibility testing method.