Hugh Leifson Glucose Broth

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Hugh Leifson Glucose Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................30.0g
Glucose.......................................................................................10.0g
Agar..............................................................................................3.0g

Peptone .........................................................................................2.0g

Yeast extract..................................................................................0.5g

Bromcresol Purple ....................................................................0.015g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Adjust pH to 7.4. Distibute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and differentiation of bacteria based on their
ability to ferment glucose. Bacteria that ferment glucose turn the
medium yellow.