Acetomicrobium faecalis Medium

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetomicrobium faecalis Medium
Composition per 1010.0mL:
NaHCO 3 ........................................................................................6.0g
Sodium acetate..............................................................................5.0g
Glucose.........................................................................................4.0g
Pancreatic digest of casein............................................................2.0g
Yeast extract..................................................................................2.0g
L -Cysteine·HCl .............................................................................0.5g
NaCl..............................................................................................0.5g
K 2 HPO 4 .....................................................................................0.225g
KH 2 PO 4 .....................................................................................0.225g
(NH 4 ) 2 SO 4 ................................................................................0.225g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O ................................................................................0.07g
Resazurin ...................................................................................1.0mg
Trace elements solution...........................................................10.0mL
Vitamin solution.......................................................................10.0mL
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
CaCl 2 ·2H 2 O..................................................................................1.0g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
FeSO 4 ·7H 2 O.................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution : Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add distilled/deionized water to 1.0L. Add remain-
ing components. Mix thoroughly. Adjust pH to 7.0 with KOH.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Calcium  DL -pantothenate...........................................................5.0mg
Lipoic acid .................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Thiamine·HCl ............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solution,
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Boil for a few minutes. Allow to cool
to room temperature under 80% N 2 + 20% CO 2 . Distribute into tubes or
flasks under 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to room temperature. Before inoculation, aseptically
and anaerobically add vitamin solution.
Use:  For the cultivation and maintenance of Acetomicrobium faecalis.