Kupferberg Trichomonas Broth

[所属分类:培养基配方] [发布时间:2021-4-25] [发布人:李佳颐] [阅读次数:] [返回]

Kupferberg Trichomonas Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Enzymatic digest of protein........................................................20.0g
L -Cysteine·HCl·H 2 O.....................................................................1.5g
Agar..............................................................................................1.0g
Maltose .........................................................................................1.0g
Chloramphenicol...........................................................................0.1g
Methylene Blue..........................................................................3.0mg
Bovine serum...........................................................................50.0mL
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except bovine serum,
to distilled/deionized water and bring volume to 950.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add bovine serum. If
desired, additional selectivity can be obtained by aseptically adding
250,000U penicillin and 1.0g streptomycin or 1.0g chloramphenicol.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use:  For the cultivation of the Trichomonas species from clinical speci-
mens.