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Lactobacilli Broth, AOAC (Lactobacilli Broth, Association of Official Analytical Chemists)
[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]
Lactobacilli Broth, AOAC
(Lactobacilli Broth, Association of
Official Analytical Chemists)
Glucose .......................................................................................10.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................1.0g
Tomato juice...........................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and preparation of inocula of stock cultures of
Lactobacillus casei ATCC 7469, Lactobacillus fermentum ATCC
9338, Lactobacillus leichmannii ATCC 4797, and Lactobacillus viri-
descens ATCC 12706 used in the microbiological assay of vitamins.
(Lactobacilli Broth, Association of
Official Analytical Chemists)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Milk, peptonized.........................................................................15.0gGlucose .......................................................................................10.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................1.0g
Tomato juice...........................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and preparation of inocula of stock cultures of
Lactobacillus casei ATCC 7469, Lactobacillus fermentum ATCC
9338, Lactobacillus leichmannii ATCC 4797, and Lactobacillus viri-
descens ATCC 12706 used in the microbiological assay of vitamins.
