Lactobacillus Chloramphenicol Agar 1

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Chloramphenicol Agar 1

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Milk, peptonized.........................................................................15.0g
Agar ............................................................................................10.0g
Glucose .......................................................................................10.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................1.0g
Chloramphenicol...........................................................................0.3g
Tomato juice ..........................................................................100.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Al-
low tubes to cool in an upright position.
Use: For the cultivation of Lactobacillus casei.