Lactobacillus Chloramphenicol Medium 2

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Chloramphenicol Medium 2

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................18.5g
Agar............................................................................................13.5g
Pancreatic digest of gelatin.........................................................10.0g
Beef extract...................................................................................8.0g
Yeast extract..................................................................................4.0g
Sodium acetate..............................................................................3.0g
K 2 HPO 4 .........................................................................................2.0g
Ammonium citrate........................................................................2.0g
Polysorbate 80 ..............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Chloramphenicol...........................................................................0.1g
MnSO 4 ·4H 2 O..............................................................................0.05g
pH 6.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation of Lactobacillus casei and Pediococcus acidi-
lactici.