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Lactobacillus Heteroferm Screen Broth (MRS, Modified)

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Heteroferm Screen Broth
(MRS, Modified)
山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Proteose peptone No. 3...............................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
2-Phenylethyl alcohol...................................................................3.0g
Ammonium citrate........................................................................2.0g
K 2 HPO 4 .........................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·H 2 O................................................................................0.05g
Bromcresol Green.......................................................................0.04g
Cycloheximide...........................................................................4.0mg
Tween™ 80................................................................................1.0mL
pH 4.3 ± 0.01 at 25°C
Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.3 with
concentrated HCl. Distribute into test tubes that contain an inverted
Durham tube. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of Lactobacillus species from
foods.

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Lactobacillus Heteroferm Screen Broth (MRS, Modified)