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Lactobacillus Orotic Acid Medium
[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]
Lactobacillus Orotic Acid Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Glucose.......................................................................................10.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................1.0g
Orotic acid ...............................................................................25.0mg
D -Pantothenic acid.....................................................................0.2mg
Tomato juice ..........................................................................100.0mL
L -Cysteine·HCl·H 2 O solution....................................................7.5mL
pH 6.8 ± 0.2 at 25°C
L -Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O...................................................................0.15g
Preparation of L -Cysteine·HCl·H 2 O Solution: Add L -cyste-
ine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into bottles in
20.0mL volumes. Add 0.15mL of L -cysteine·HCl·H 2 O solution to each
bottle containing 20.0mL of medium. Autoclave for 15 min at 15 psi
pressure–121°C. Screw caps tightly to maintain reduced conditions.
Use: For the cultivation of Lactobacillus helveticus.
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Milk, peptonized.........................................................................15.0gGlucose.......................................................................................10.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Sorbitan monooleate complex ......................................................1.0g
Orotic acid ...............................................................................25.0mg
D -Pantothenic acid.....................................................................0.2mg
Tomato juice ..........................................................................100.0mL
L -Cysteine·HCl·H 2 O solution....................................................7.5mL
pH 6.8 ± 0.2 at 25°C
L -Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O...................................................................0.15g
Preparation of L -Cysteine·HCl·H 2 O Solution: Add L -cyste-
ine·HCl·H 2 O to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 2–3 min. Distribute into bottles in
20.0mL volumes. Add 0.15mL of L -cysteine·HCl·H 2 O solution to each
bottle containing 20.0mL of medium. Autoclave for 15 min at 15 psi
pressure–121°C. Screw caps tightly to maintain reduced conditions.
Use: For the cultivation of Lactobacillus helveticus.
