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Lactobacillus rimae Medium with Tween™

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus rimae Medium with Tween™
山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................10.0g
Peptone..........................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Glucose .........................................................................................5.0g
(NH 4 ) 2 SO 4 .....................................................................................0.5g
L -Cysteine·HCl..............................................................................0.5g
Tween™ 80...................................................................................0.2g
Resazurin ...................................................................................1.0mg
Mineral solution.......................................................................40.0mL
Fatty acid mixture......................................................................3.1mL
Hemin solution...........................................................................0.5mL
Vitamin K 1 .................................................................................0.2mL
pH 6.9 ± 0.2 at 25°C
Mineral Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.48g
CaCl 2 ·2H 2 O...................................................................................0.3g
Preparation of Mineral Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Fatty Acid Mixture:
Composition per 31.0mL:
Acetic acid...............................................................................17.0mL
Propionic acid............................................................................6.0mL
n-Butyric acid ............................................................................4.0mL
n-Valeric acid.............................................................................1.0mL
iso-Valeric acid ..........................................................................1.0mL
iso-Butyric acid..........................................................................1.0mL
DL -2-Methylbutyric acid............................................................1.0mL
Preparation of Fatty Acid Mixture: Combine components. Mix
thoroughly. Adjust pH to 7.5 with concentrated NaOH.
Hemin Solution:
Composition per 1.0mL:
Hemin ........................................................................................5.0mg
NaOH (1N solution)...................................................................1.0mL
Preparation of Hemin Solution: Add hemin to 1.0mL of NaOH
solution. Mix thoroughly.
Preparation of Medium: Add components, except L -cysteine·HCl,
hemin solution, and fatty acid mixture, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Continue boiling for 5 min. Cool to room temperature while sparg-
ing with 100% CO 2 . Add L -cysteine·HCl, hemin solution, and fatty
acid mixture. Adjust pH to 6.9 with 8N NaOH while continuing to
sparge with 100% CO 2 . After pH has been reached, sparge with 100%
N 2 . Anaerobically distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Lactobacillus rimae and
Lactobacillus uli.

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Lactobacillus rimae Medium with Tween™