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Lactobacillus Selection HiVeg Agar Base with Acetic Acid and Polysorbate
[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]
Lactobacillus Selection HiVeg Agar Base
with Acetic Acid and Polysorbate
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Glucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Plant hydrolysate.........................................................................10.0g
KH 2 PO 4 .........................................................................................6.0g
Yeast extract..................................................................................5.0g
Ammonium citrate........................................................................2.0g
MgSO 4 ......................................................................................0.575g
FeSO 4 ........................................................................................0.034g
MnSO 4 ........................................................................................0.12g
Acetic acid, glacial..................................................................1.32 mL
Polysorbate 80............................................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Source: This medium, without polysorbate 80 or glacial acetic acid,
is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L.Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 5.5. Do not autoclave. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and enumeration of lacto-
bacilli from foods.
with Acetic Acid and Polysorbate
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Sodium acetate·3H 2 O..................................................................25.0gGlucose .......................................................................................20.0g
Agar ............................................................................................15.0g
Plant hydrolysate.........................................................................10.0g
KH 2 PO 4 .........................................................................................6.0g
Yeast extract..................................................................................5.0g
Ammonium citrate........................................................................2.0g
MgSO 4 ......................................................................................0.575g
FeSO 4 ........................................................................................0.034g
MnSO 4 ........................................................................................0.12g
Acetic acid, glacial..................................................................1.32 mL
Polysorbate 80............................................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Source: This medium, without polysorbate 80 or glacial acetic acid,
is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L.Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 5.5. Do not autoclave. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the selective isolation, cultivation, and enumeration of lacto-
bacilli from foods.
