Lauryl Sulfate Broth, Fluorocult (Fluorocult Lauryl Sulfate Broth)

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lauryl Sulfate Broth, Fluorocult
(Fluorocult Lauryl Sulfate Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ......................................................................................20.0g
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................2.75g
KH 2 PO 4 .......................................................................................2.75g
L -tryptophan....................................................................................1.g
Sodium lauryl sulfate....................................................................0.1g
4-Methylumbelliferyl-ß-D-glucuronide........................................0.1g
pH 6.8 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium:  Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Cool. Distribute into test tubes containing inverted Durham tubes.
Autoclave for 15 min at 15 psi pressure–121°C. The prepared broth is
clear and yellowish-brown.
Use:  For the detection of E. coli in milk. The medium complies with
the German-DIN-Norm 10183 for the examination of milk, with the
regulations acc. to § 35 LMBG (01.00/54) for the examination of food,
and according to ISO/DIS 11886-2.2 (1994) for milk and milk prod-
ucts. The lauryl sulfate largely inhibits the growth of undesirable
microbial flora. The presence of E. coli is indicated by fluorescence
under a long wavelength UV lamp. A positive indole reaction and gas
formation due to fermentation of lactose confirm the results.