Acid Tomato Broth

[所属分类:培养基配方] [发布时间:2019-9-20] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acid Tomato Broth
Composition per liter:
Glucose.......................................................................................10.0g
Peptone .......................................................................................10.0g
Yeast extract..................................................................................5.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
Tomato juice ..........................................................................250.0mL
L -Cysteine solution....................................................................0.5mL
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine.....................................................................................0.1g
Preparation of  L -Cysteine Solution: Add 0.1g of  L -cysteine to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except  L -cysteine so-
lution, to distilled/deionized water and bring volume to 999.5mL. Mix
thoroughly. Adjust pH to 4.8. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 0.5mL of sterile  L -cysteine solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of a variety of fungi.