MacConkey Agar, CS

[所属分类:培养基配方] [发布时间:2021-5-11] [发布人:网站管理员2] [阅读次数:] [返回]

MacConkey Agar, CS

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................17.0g
Agar ............................................................................................13.5g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Proteose peptone...........................................................................3.0g
Bile salts........................................................................................1.5g
Neutral Red.................................................................................0.03g
Crystal Violet.............................................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a prepared medium from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and differentiation of lactose-fermenting and
lactose-nonfermenting Gram-negative bacteria while also controlling
the swarming of Proteus species, if present. Lactose-fermenting organ-
isms appear as red to pink colonies. Lactose-nonfermenting organisms
appear as colorless or transparent colonies.