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Malt Yeast Extract 50% Glucose Agar (MY50G) (ATCC Medium 2093)

[所属分类:培养基配方] [发布时间:2021-5-13] [发布人:网站管理员2] [阅读次数:] [返回]
Malt Yeast Extract 50% Glucose Agar (MY50G)
(ATCC Medium 2093)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.....................................................................................500.0g
Agar............................................................................................10.0g
Malt extract.................................................................................10.0g
Yeast extract..................................................................................2.5g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium:  Add agar, yeast extract, and malt extract
to distilled/deionized water and bring volume to 500mL. Mix thor-
oughly. Gently heat while stirring until boiling. Slowly add glucose
while stirring to avoid lumps. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Do not overheat or agar will not
harden. Pour into sterile Petri dishes or leave in tubes. Note: This agar
hardens very slowly.
Use: For the cultivation of yeasts and molds.
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