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Malt 4% Yeast Extract Agar with Lupine Stems (MYA4 with Lupine Stems)

[所属分类:培养基配方] [发布时间:2021-5-13] [发布人:网站管理员2] [阅读次数:] [返回]

Malt 4% Yeast Extract Agar with Lupine Stems
(MYA4 with Lupine Stems)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Malt extract.................................................................................40.0g
Agar ............................................................................................20.0g
Yeast extract..................................................................................1.0g
Lupine stems...........................................................................variable
Preparation of Medium: Add components, except lupine stems, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes.
Cut lupine stems into 8.0cm-long pieces. Add 2–3 lupine stems per
tube. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to
cool in a slanted position.
Use: For the cultivation of Ceratocystiopsis minuta-bicolor, Ceratocysti-
opsis retusi, Ceratocystis pilifera, Ceratocystis olivaceapini, Ceratocystis
multiannulata, Ceratocystis nigra, Ceratocystis olivacea, Ceratocystis
tremuloaurea, Chaetomium indicum, and Chaetospermum chaetosporum.

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Malt 4% Yeast Extract Agar with Lupine Stems (MYA4 with Lupine Stems)