Manganese Agar No. 1 (Mn Agar No. 1)

[所属分类:培养基配方] [发布时间:2021-5-13] [发布人:网站管理员2] [阅读次数:] [返回]

Manganese Agar No. 1
(Mn Agar No. 1)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
MnCO 3 ..........................................................................................2.0g
Beef extract...................................................................................1.0g
Fe(NH 4 ) 2 (SO 4 ) 2 ..........................................................................0.15g
Sodium citrate.............................................................................0.15g
Yeast extract..............................................................................0.075g
Cyanocobalamin solution ........................................................10.0mL
Cyanocobalamin Solution:
Composition per 10.0mL:
Cyanocobalamin....................................................................0.005mg
Preparation of Cyanocobalamin Solution:  Add cyanocobala-
min to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except cyanocobala-
min, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 10.0mL of the sterile cyanocobalamin solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the isolation and cultivation of iron and sulfur bacteria. Also
used to differentiate Leptothrix (Sphaerotilus) discophorus from Spha-
erotilus natans.