Manganese Agar No. 2 (Mn Agar No. 2)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Manganese Agar No. 2
(Mn Agar No. 2)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
MnSO 4 ·H 2 O.............................................................................10.0mg
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use
freshly prepared solution.
Use: For the enumeration, enrichment, and isolation of iron and sulfur
bacteria. For the isolation and cultivation of Leptothrix species from
water.
Manganese Medium for Pseudomonas species
Composition per liter:
Noble agar...................................................................................10.0g
MnCO 3 ..........................................................................................1.0g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O ................................................................0.15g
Sodium citrate.............................................................................0.15g
Yeast extract..............................................................................0.075g
Na 4 P 2 O 7 ·10H 2 O..........................................................................0.05g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Pseudomonas putida and
other Pseudomonas species.