Mannitol Salt Broth

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Salt Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................100.0g
Pancreatic digest of casein..........................................................17.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
D- Mannitol ....................................................................................2.5g
Phenol Red................................................................................0.025g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the selective isolation and cultivation of staphylococci from
foods and nonclinical specimens. Mannitol-utilizing organisms turn
the medium yellow.