Mannitol Yolk Polymyxin Agar (MYP Agar)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Yolk Polymyxin Agar
(MYP Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 110.0mL:

Agar..............................................................................................1.5g
NaCl..............................................................................................1.0g
Peptone .........................................................................................1.0g
D- Mannitol ....................................................................................1.0g
(NH 4 ) 2 PO 4 ....................................................................................0.1g
Meat extract..................................................................................0.1g
Phenol Red.................................................................................2.5mg
Egg yolk emulsion, 20%..........................................................10.0mL
Polymyxin B solution................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Egg Yolk Emulsion, 20%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 20%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 20.0mL of egg yolk emulsion and add to 80.0mL of 0.9%
NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-

ter sterilize.

Preparation of Medium: Add components—except egg yolk emul-
sion, 20%, and polymyxin B solution—to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL of sterile egg yolk emulsion, 20%, and 1.0mL
of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri
dishes.
Use: For the cultivation and maintenance of Bacillus cereus.