Marine Spirochete Medium (DSMZ Medium 1008)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marine Spirochete Medium
(DSMZ Medium 1008)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Trypticase peptone........................................................................2.0g
Yeast extract .................................................................................1.0g
Na-thioglycolate ..........................................................................1.0g
Resazurin ..................................................................................0.5mg
Charcoal-filtered, natural seawater .......................................800.0mL
Cellobiose solution..................................................................20.0mL
pH 7.5 ± 0.2 at 25°C
Cellobiose Solution :
Composition per 100.0mL:
Cellobiose...................................................................................10.0g
Preparation of Cellobiose Solution:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Sparge with 100% N 2 . Filter sterilize.
Preparation of Medium: Add components, except thioglycolate
and cellobiose solution, to seawater and bring volume to 800.0mL. Mix
thoroughly. Bring volume to 980.0mL with distilled/deionized water.
(Note: Bottled water from Biomaris GmbH can be used instead of fil-
tered seawater.) Gently heat and bring to boiling. Boil for 3 min. Cool
to room temperature while sparging with 100% N 2 . Add the thioglyco-
late. Adjust pH to 7.5 with 10N NaOH. Dispense into tubes or bottles
under 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically and anoxically add cellobiose solu-
tion.
Use: For the cultivation of Spirochaeta bajacaliforniensis.