Marinobacter Medium (DSMZ Medium 941)

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Marinobacter Medium
(DSMZ Medium 941)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................6.0g
NH 4 Cl ...........................................................................................1.0g
Na-acetate .....................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
KCl................................................................................................0.1g
KH 2 PO 4 .........................................................................................0.1g
Peptone..........................................................................................0.1g
CaCl 2 ·2H 2 O.................................................................................0.04g
Trace elements solution SL-7 ....................................................1.0mL
Vitamin solution, concentrated..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·7H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ...........................................................................190.0mg
MnCl 2 ·4H 2 O...........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ......................................................................................62.0mg
CuCl 2 ·2H 2 O .............................................................................17.0mg
HCl (25% solution)....................................................................6.5mL
Preparation of Trace Elements Solution SL-7: Add FeCl 2 ·7H 2 O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15
psi pressure–121°C.
Vitamin Solution, Concentrated:
Composition per 100.0mL:
Pyridoxine-HCl........................................................................10.0mg
Thiamine-HCl·2H 2 O..................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
D -Ca-pantothenate......................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Lipoic acid.................................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Vitamin B 12 ................................................................................0.1mg
Preparation of Vitamin Solution, Concentrated:  Add compo-
nents to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Marionobacter sp.