Martin-Lewis Agar

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

Martin-Lewis Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
Hemoglobin ................................................................................10.0g
Pancreatic digest of casein............................................................7.5g
Selected meat peptone ..................................................................7.5g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Cornstarch.....................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
Supplement solution ................................................................10.0mL
VCAT inhibitor........................................................................10.0mL
pH 7.2 ± 0.22 at 25°C
Source:  Martin-Lewis agar is available as a prepared medium from
BD Diagnostic Systems.
Supplement Solution:
Composition per liter:
Glucose .....................................................................................100.0g
L- Cysteine·HCl............................................................................25.9g
L- Glutamine.................................................................................10.0g
L- Cystine .......................................................................................1.1g
Adenine.........................................................................................1.0g
Nicotinamide adenine dinucleotide ............................................0.25g
Vitamin B 12 ...................................................................................0.1g
Thiamine pyrophosphate...............................................................0.1g
Guanine·HCl...............................................................................0.03g
Fe(NO 3 ) 3 ·6H 2 O...........................................................................0.02g
p-Aminobenzoic acid................................................................0.013g

Thiamine·HCl ............................................................................3.0mg

Source: The supplement solution IsoVitaleX ® enrichment is available
from BD Diagnostic Systems. This enrichment may be replaced by
supplement VX from BD Diagnostic Systems.
Preparation of Supplement Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
VCAT Inhibitor:
Composition per 10.0mL:
Colistin.......................................................................................7.5mg
Trimethoprim lactate..................................................................5.0mg
Vancomycin...............................................................................4.0mg
Anisomycin.................................................................................0.02g
Preparation of VCAT Inhibitor: Add components to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except supplement so-
lution and VCAT inhibitor, to distilled/deionized water and bring vol-
ume to 980.0mL. Gently heat while stirring and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile supplement solution and sterile VCAT inhibitor.
Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of pathogenic Neisseria from
specimens containing mixed flora of bacteria and fungi.