M-Bismuth Sulfite HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-5-17] [发布人:网站管理员2] [阅读次数:] [返回]

M-Bismuth Sulfite HiVeg Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................20.0g
Bismuth sulfite indicator.............................................................16.0g
Glucose.......................................................................................10.0g
Plant extract................................................................................10.0g
Na 2 HPO 4 .......................................................................................8.0g
FeSO 4 ............................................................................................0.6g
Brilliant Green............................................................................0.05g
pH 7.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly and heat with frequent
agitation until boiling. Boil for 1 min. Do not autoclave. Cool to 45°–
50°C. Mix to disperse the precipitate and aseptically distribute into
sterile tubes or flasks. Use 2.0–2.2mL of medium for each membrane
filter.
Use: For the selective isolation of Salmonella typhi and other enteric
bacilli and for the detection of Salmonella by the membrane filter
method.