McBride Agar, Modified

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

McBride Agar, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glycine anhydride.......................................................................10.0g
Tryptose ......................................................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................3.0g
Phenylethanol................................................................................2.5g
LiCl...............................................................................................0.5g
Cycloheximide solution...........................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide..............................................................................0.2g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-

mation and inhalation.

Preparation of Medium: Add components, except cycloheximide
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the isolation of Listeria monocytogenes from dairy products.