McClung-Toabe Agar, Modified

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

McClung-Toabe Agar, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 2...............................................................40.0g
Agar ............................................................................................20.0g
Na 2 HPO 4 .......................................................................................5.0g
Glucose .........................................................................................2.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Egg yolk emulsion, 50%........................................................100.0mL
Hemin solution...........................................................................1.0mL
pH 7.6 ± 0.2 at 25°C
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Hemin Solution:
Composition per 100.0mL:
Hemin............................................................................................0.5g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium: Add components, except egg yolk emul-
sion, 50%, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat while stirring and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti-
cally add 100.0mL of sterile egg yolk emulsion, 50%. Mix thoroughly.
Pour into sterile Petri dishes in 20.0mL volumes.
Use: For the cultivation of a wide variety of anaerobic bacteria. For the
differentiation of anaerobic bacteria based on lecithinase production
and lipase production. Bacteria that produce lecithinase appear as col-
onies surrounded by a zone of insoluble precipitate. Bacteria that pro-
duce lipase appear as colonies with a pearly iridescent sheen.