Me15% MH Medium (DSMZ Medium 582)

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Me15% MH Medium
(DSMZ Medium 582)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..........................................................................................121.5g
MgSO 4 ........................................................................................14.4g
MgCl 2 ..........................................................................................10.5g
Yeast extract................................................................................10.0g
Proteose peptone no. 3..................................................................5.0g
KCl................................................................................................3.0g
Glucose.........................................................................................1.0g
CaCl 2 ..........................................................................................0.54g
NaBr..........................................................................................0.039g
NaHCO 3  solution.....................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ........................................................................................0.9g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 10.0mL NaHCO 3 solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Bacillus halophilus.