MED IIa

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

MED IIa

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tris buffer stock solution.........................................................10.0mL
CaCl 2 (5.0% solution)..............................................................10.0mL
MgSO 4 ·7H 2 O (3.33% solution).................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Tris Buffer Stock Solution:
Composition per 500.0mL:
Tris(hydroxymethyl)aminomethane·HCl..................................35.01g
Tris(hydroxymethyl)aminomethane ...........................................3.35g
Preparation of Tris Buffer Stock Solution: Add components to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly. Adjust pH to 7.2.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 20 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Vampirovibrio chlorellavo-
rus.