Medium A for Producing Lysates

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium A for Producing Lysates

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Nutrient broth................................................................................8.0g
KCl................................................................................................1.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
MnCl 2 .......................................................................................1.25mg
FeSO 4 (1.0mM solution)............................................................1.0mL
Ca(NO 3 ) 2 (1.0M solution)..........................................................1.0mL
pH 7.0–7.2 at 25°C
Preparation of Medium:  Add components, except FeSO 4 and
Ca(NO 3 ) 2 , to distilled/deionized water and bring volume to 998.0mL.
Mix thoroughly. Adjust pH to 7.0–7.2. Autoclave for 30 min at 15 psi
pressure–115°C. Cool to 45°–50°C. Prepare 1.0mM FeSO 4 solution
and 1.0M Ca(NO 3 ) 2 solution separately. Filter sterilize both solutions.
Aseptically add the sterile FeSO 4 solution and sterile Ca(NO 3 ) 2 solu-
tion to the cooled sterile basal medium. Mix thoroughly. Distribute into
sterile tubes or flasks.
Use:  For the cultivation of microorganisms to be lysed.