Medium for Bacillus stearothermophilus

[所属分类:培养基配方] [发布时间:2021-5-18] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Bacillus stearothermophilus

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

NH 4 Cl ...........................................................................................1.0g
K 2 HPO 4 .........................................................................................0.5g
Yeast extract..................................................................................0.2g
Casamino acids.............................................................................0.1g
MgSO 4 ·7H 2 O..............................................................................0.02g
Phenol solution ......................................................................100.0mL
Trace elements solution SL-4....................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Phenol Solution:
Composition per 100.0mL:
Phenol.........................................................................................0.47g
Preparation of Phenol Solution:  Add phenol to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................0.5g
FeSO 4 ·7H 2 O................................................................................. 0.2g
Trace elements solution SL-6................................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O .................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Trace Elements Solution SL-4:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except phenol solution
and trace elements solution SL-4, to distilled/deionized water and
bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Aseptically add 100.0mL of sterile phenol solu-
tion and 1.0mL of sterile trace elements solution SL-4. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation of Bacillus stearothermophilus.