Medium for Chlorate Respirers (DSMZ Medium 908)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Chlorate Respirers
(DSMZ Medium 908)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A.....................................................................................1.0L
Solution B................................................................................10.0mL
Solution C................................................................................10.0mL
Vitamin solution.........................................................................5.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Solution A:
Composition per liter:
NaHCO 3 ........................................................................................2.5g
Na-acetate...................................................................................1.36g
NaClO 3 .........................................................................................1.0g
NaH 2 PO 4 .......................................................................................0.6g
NH 4 Cl .........................................................................................0.25g
KCl................................................................................................0.1g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% N 2
+ 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature.
Solution B:
Composition per 10.0mL:
MgSO 4 .....................................................................................30.0mg
CaCl 2 ·2H 2 O .............................................................................10.0mg
Preparation of Solution B:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80%
N 2  + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Solution C:
Na 2 MoO 4 .................................................................................25.0mg
Na 2 WO 4 ·2H 2 O.........................................................................25.0mg
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80%
N 2  + 20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Vitamin Solution:
Composition per liter:
Vitamin B 12 ..............................................................................50.0mg
Pantothenic acid.......................................................................50.0mg
Riboflavin................................................................................50.0mg
Alpha-lipoic acid .....................................................................50.0mg
p-Aminobenzoic acid...............................................................50.0mg
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................25.0mg
Nicotinamide............................................................................25.0mg
Biotin.......................................................................................20.0mg
Folic acid .................................................................................20.0mg
Pyridoxamine-HCl...................................................................10.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
H 3 BO 3 ....................................................................................300.0mg
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
HCl (25% solution)....................................................................7.7mL
Preparation of Trace Elements Solution SL-10: Add
FeCl 2 ·4H 2 O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/
deionized water and bring volume to 1.0L. Add remaining compo-
nents. Mix thoroughly. Sparge with 100% N 2 . Autoclave for 15 min at
15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Aseptically and anaerobically combine
1000.0mL solution A, 10.0mL solution B and 10.0mL solution C.
Aseptically and anaerobically add 5.0mL vitamin solution and 1.0mL
trace elements solution SL-10. Mix thoroughly. The pH should be 7.2.
Use: For the cultivation of Dechloromonas agitata and Azospira
oryzae.