Medium D for Sulfate Reducers (Postgate’s Medium D for Sulfate Reducers)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium D for Sulfate Reducers
(Postgate’s Medium D for Sulfate Reducers)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium pyruvate...........................................................................3.5g
MgCl 2 ·6H 2 O..................................................................................1.6g
NH 4 Cl ...........................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..............................................................................0.004g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Malate or fumarate may also be used
as a carbon source. For marine bacteria, NaCl may be added or seawa-
ter used in place of distilled/deionized water. Mix thoroughly. Adjust
pH to 7.5. Filter sterilize. Aseptically distribute into sterile tubes or

flasks.

Use:  For the cultivation of Desulfovibrio species and Desulfotomacu-
lum species that can grow in the absence of sulfate.