Medium for DSM 14457 and DSM 14458 (DSMZ Medium 956)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for DSM 14457 and DSM 14458
(DSMZ Medium 956)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
NaCl..............................................................................................0.5g
MgSO 4 ·7H 2 O............................................................................0.125g
FeSO 4 ·7H 2 O................................................................................0.02g
Methanol solution....................................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Methanol Solution:
Composition per 10.0mL:
Methanol....................................................................................5.0mL
Preparation of Methanol Solution: Add methanol to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except methanol solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
room temperature. Aseptically add 10.0mL sterile methanol solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Methylobacterium lusitanum and Methy-
lobacterium suomiense.