Medium K (DSMZ Medium 1122)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium K
(DSMZ Medium 1122)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
(NH 4 ) 2 SO 4 ....................................................................................2.0g
KH 2 PO 4 ........................................................................................2.0g
NaCl..............................................................................................0.5g
MgSO 4 · 7H 2 O............................................................................0.125g
FeSO 4 · 7H 2 O..............................................................................0.002g
Methanol, sterilized by filtration .............................................10.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except methanol, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 7.2 0. Gently heat while stirring and bring to boiling.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C. Aseptically add 10.0 sterile methanol. Mix thoroughly. Pour
into Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation of Methylobacillus pratensis and Methylovo-
rus mays.